Fructosyltransferase yog dab tsi?
Fructosyltransferases (FTs) yog ib pab pawg tseem ceeb ntawm cov enzymes koom nrog hauv biosynthesis ntawm fructans hauv qee cov nroj tsuag thiab hauv ntau cov kab mob. Ntau qhov kev tshawb fawb tau pom tias FTs ua lub luag haujlwm tseem ceeb hauv kev tswj hwm qib ntawm fructan molecules thaum lub sij hawm abiotic kev nyuaj siab rau qee hom nroj tsuag. Vim tias lawv muaj ntau hom kev ua haujlwm zoo, kev siv zog tau nce ntxiv los txhim kho cov ntawv thov fructans hauv cov zaub mov zoo thiab ntxiv cov txiaj ntsig kev noj qab haus huv ntawm cov nroj tsuag. Rau cov laj thawj no, kev txaus siab rau kev tshawb nrhiav ncaj qha thiab tus nqi ntau dua - txoj hauv kev zoo rau kev tsim cov fructans kuj tau nce ntxiv. Hauv daim ntawv no, peb tshuaj xyuas cov kev tshawb fawb tsis ntev los no ntawm cov nroj tsuag FTs thiab xaus lawv lub luag haujlwm hauv kev tswj hwm ntawm fructan biosynthesis thiab lawv siv los tsim fructans ntawm kev lag luam. Peb kuj nthuav qhia cov kev cov nyom thiab cov cib fim uas FTs yuav nthuav tawm yav tom ntej.
Txhua Yam Koj Yuav Tsum Paub
Lub luag haujlwm ntawm Fructooligosaccharides yog dab tsi?
Ntawm cov no, fructooligosaccharides (FOS) ua lub luag haujlwm tseem ceeb hauv cov txheej txheem hauv lub plab zom mov, pab tsim cov khoom luv luv -cov roj fatty acids los ntawm cov kab mob muaj txiaj ntsig, yog li txhawb lawv txoj kev loj hlob.
Dab tsi yog invertase enzyme siv rau?
Invertase yog ib qho ntawm cov khoom xyaw zais cia hauv cov khoom qab zib- ua kev lag luam. Nws yog ib qho enzyme uas feem ntau siv los ua cov khoom qab zib ua kua, chocolate - npog cherries, fondant candies, creme qe, thiab lwm yam cordials. Invertase feem ntau yog muab los ntawm cov poov xab, xws li los ntawm cov chaw ua mov ci lossis npias breweries.
Fos ua li cas?
Kev tsim tawm ntawm FOS ntawm kev lag luam nplai yog ua tiav los ntawm tshuaj lom neeg lossis enzymatic hydrolysis mediated los ntawm inulin lossis sucrose transfructosylation.
Dab tsi enzyme zom fructose hauv lub cev?
Kev zom ntawm fructose hauv lub cev Fructose yog nqus mus rau hauv cov hlab ntsha hauv cov hnyuv nrog kev pab los ntawm kev thauj cov protein. Lub plab zom mov enzyme Xylose Isomerase hloov fructose dawb rau hauv qabzib, ua kom muaj qhov sib piv 1: 1 ntawm cov piam thaj thiab fructose, tso cai rau ob qho tib si kom nqus tau zoo hauv cov hnyuv.
Cov ntsiab lus tseem ceeb:
Fructooligosaccharides (FOS) kuj qee zaum hu ua oligofructose lossis oligofructan, yog oligosaccharide fructans, siv los ua lwm yam khoom qab zib. FOS nthuav tawm cov qib qab zib ntawm 30 thiab 50 feem pua ntawm cov suab thaj hauv cov khoom lag luam npaj syrups.
Lub enzyme uas hloov cov piam thaj (sucrose) rau hauv qabzib thiab fructose yog Invertase. Cov txheej txheem no hu ua hydrolysis ntawm sucrose thiab yog catalyzed los ntawm invertase, uas tseem hu ua sucrase los yog beta-fructofuranosidase.
Cov neeg uas muaj kab mob plab zom mov xws li chim siab plob tsis so tswj (IBS) yuav xav kom tsis txhob siv FOS, vim tias lawv lub plab zom mov yuav tsis teb zoo rau cov teebmeem.
Muaj nuj nqi:FTase catalyzes hydrolysis ntawm sucrose thiab hloov pauv ntawm fructose residues los ua FOS (xws li 1-kestose), ua raws li biocatalyst hauv kev lag luam zaub mov.
Daim ntawv thov:Siv los tsim cov khoom muaj txiaj ntsig zoo -FOS prebiotic, uas tau xav tau ntau ntxiv hauv cov khoom noj thiab cov khoom noj khoom haus.
ANALYTICAL RESULTS
| Cov khoom kuaj | Method / Ref. | Specification | Kev tshwm sim | Xaus |
|---|---|---|---|---|
| Kev txheeb xyuas | Hauv -house ID / daim ntawv thov | Fructosyltransferase (EC 2.4.1.9) | Ua raws | Hla |
| Qhov tshwm sim | Pom kev | Dawb rau tawm -dawb hmoov | Tawm- hmoov dawb | Hla |
| Kev ua enzyme | Fructosyltransferase kev soj ntsuam / kev xa khoom | NLT 5,000 U/g | 5,100 U/g | Hla |
| Lub luag haujlwm Unit txhais | Tus neeg muab khoom siv -txhais chaw | Qhia xwb | U/g qhia | Hla |
| pH (1% tov) | pH ntsuas | 4.5 – 6.5 | 5.3 | Hla |
| Poob rau ziab | Gravimetric / USP 731 | Tsawg dua lossis sib npaug li 8.0% | 4.2% | Hla |
| Lead (Pb) | ICP-MS / ICP-OES | Tsawg dua lossis sib npaug li 5.0 mg / kg | 0.18 mg/kg | Hla |
| Arsenic (As) | ICP-MS / ICP-OES | Tsawg dua lossis sib npaug li 3.0 mg / kg | 0.09 mg/kg | Hla |
| Cadmium (Cd) | ICP-MS / ICP-OES | Tsawg dua lossis sib npaug li 0.5 mg / kg | < 0.05 mg/kg | Hla |
| Mercury (Hg) | ICP-MS / ICP-OES | Tsawg dua lossis sib npaug li 0.5 mg / kg | < 0.01 mg/kg | Hla |
| Tag nrho cov phaj suav | Phaj suav | Tsawg dua lossis sib npaug li 50,000 CFU / g | 2.0 × 10³ CFU/g | Hla |
| Coliforms | Phaj suav | Tsawg dua lossis sib npaug li 30 CFU / g | < 10 CFU/g | Hla |
| E. coli | Tsis Muaj Kev Xeem | Tsis zoo / 25g | Tsis Pom | Hla |
| Salmonella | Tsis Muaj Kev Xeem | Tsis zoo / 25g | Tsis Pom | Hla |
Txheej txheem ntau lawm
