Phospholipase yog dab tsi?
Phospholipases yog ib pab pawg neeg sib txawv ntawm lipolytic enzymes uas hydrolyze phospholipid substrates rau hauv fatty acids thiab lwm yam lipophilic tshuaj, ua lub luag haujlwm tseem ceeb hauv kev hloov cov teeb liab, kev zom zaub mov, thiab kho daim nyias nyias. Lawv tau muab faib ua hom A, B, C, thiab D raws li qhov tshwj xeeb ester daim ntawv cog lus lawv cleave, nrog PLA2 ua haujlwm ntawm sn-2 txoj haujlwm thiab PLC / PLD tsom rau phosphodiester daim ntawv cog lus.
Txhua Yam Koj Yuav Tsum Paub
Lub luag haujlwm ntawm phospholipase yog dab tsi?
Phospholipase A2s yog cov enzymes tseem ceeb hauv qhov no vim lawv lub luag haujlwm ua thawj lub tshuab hluav taws xob ntawm cov dawb polyunsaturated fatty acids, uas yog precursors ntawm ntau tsev neeg ntawm cov tebchaw ua si ntau lub luag haujlwm hauv kev mob.
PLC thiab IP3 yog dab tsi?
Phospholipase C (PLC) 1 hydrolyzes phosphatidylinositol 4,5-bisphosphate los tsim cov tub txib thib ob, inositol 1,4,5-trisphosphate (IP3) thiab diacylglycerol (DAG). IP3 induces ib ntus nce nyob rau hauv intracellular free Ca2+, thaum DAG ncaj qha activates protein kinase C.
Qhov twg phospholipase tsim nyob rau hauv tib neeg lub cev?
Qee hom phospholipase A2 yog zais cia, xws li cov phospholipases ua los ntawm cov txiav rau kev zom zaub mov. Lwm tus yog tsim nyob rau hauv cov hlwb, qhov chaw uas lawv pab nrog tsim cov signaling molecules. Txhua tus ntawm lawv sib koom ua haujlwm zoo sib xws ntawm kev txiav tawm ib qho ntawm cov tails ntawm phospholipids.
Dab tsi yog lub luag haujlwm ntawm phospholipase hauv lub plab zom mov?
Phospholipases yog cov enzymes digestive loj thiab ua lub luag haujlwm tseem ceeb hauv feem ntau cov txheej txheem physiological nrog rau cov cim ntawm ntau lub teeb liab lipids, thiab hauv kev sib sau, zoo li cuam tshuam rau txhua yam kab mob.
Cov ntsiab lus tseem ceeb:
Main Hom & Function:
Phospholipase A1 (PLA1):Cleaves lub 𝑠𝑛-1 acyl chain.
Phospholipase A2 (PLA2):Hydrolyzes 𝑠𝑛-2 txoj hauj lwm los tso tawm arachidonic acid (tus ua ntej rau cov neeg kho mob inflammatory).
Phospholipase B (PLB):Muaj ob qho kev ua PLA1 thiab PLA2.
Phospholipase C (PLC):Cleaves ua ntej pawg phosphate, tsim cov diacylglycerol (DAG) thiab inositol phosphates (qhov tseem ceeb taw qhia cov molecules).
Phospholipase D (PLD):Cleaves tom qab pab pawg phosphate, tsim phosphatidic acid.
Daim ntawv thov:
1. Txhim kho lub khob noom cookie tuav thiab ruaj khov.
2. Markedly txhim khu lub whiteness thiab crust brightness ntawm steamed qhob cij.
3. Txhim kho qhov cub lub caij nplooj ntoos hlav thiab tag nrho zoo ntawm steamed qhob cij thiab qhob cij.
4. Txo los yog hloov tag nrho cov emulsifiers.
ANALYTICAL RESULTS
| Cov khoom kuaj | Method / Ref. | Specification | Kev tshwm sim | Xaus |
|---|---|---|---|---|
| Kev txheeb xyuas | Hauv -house ID / daim ntawv thov | Phospholipase (EC 3.1.1.4) | Ua raws | Hla |
| Qhov tshwm sim | Pom kev | Dawb rau tawm -dawb hmoov | Tawm- hmoov dawb | Hla |
| Kev ua enzyme | Phospholipase assay / khoom siv | NLT 5,000 U/g | 5,300 U/g | Hla |
| Lub luag haujlwm Unit txhais | Tus neeg muab khoom siv -txhais chaw | Qhia xwb | U/g qhia | Hla |
| pH (1% tov) | pH ntsuas | 5.0 – 7.0 | 6.1 | Hla |
| Poob rau ziab | Gravimetric / USP 731 | Tsawg dua lossis sib npaug li 8.0% | 4.2% | Hla |
| Lead (Pb) | ICP-MS / ICP-OES | Tsawg dua lossis sib npaug li 5.0 mg / kg | 0.18 mg/kg | Hla |
| Arsenic (As) | ICP-MS / ICP-OES | Tsawg dua lossis sib npaug li 3.0 mg / kg | 0.09 mg/kg | Hla |
| Cadmium (Cd) | ICP-MS / ICP-OES | Tsawg dua lossis sib npaug li 0.5 mg / kg | < 0.05 mg/kg | Hla |
| Mercury (Hg) | ICP-MS / ICP-OES | Tsawg dua lossis sib npaug li 0.5 mg / kg | < 0.01 mg/kg | Hla |
| Tag nrho cov phaj suav | Phaj suav | Tsawg dua lossis sib npaug li 50,000 CFU / g | 2.0 × 10³ CFU/g | Hla |
| Coliforms | Phaj suav | Tsawg dua lossis sib npaug li 30 CFU / g | < 10 CFU/g | Hla |
| E. coli | Tsis Muaj Kev Xeem | Tsis zoo / 25g | Tsis Pom | Hla |
| Salmonella | Tsis Muaj Kev Xeem | Tsis zoo / 25g | Tsis Pom | Hla |
Txheej txheem ntau lawm
